List from the Encyclopedia of Fictional People:

Rev. Will B. Dunn (Kudzu - comic strip)
Father John Mulcahy (M*A*S*H)
Sister Bertrille (Flying Nun)
Father Guido Sarducci (SNL)
Rev. Jim Ignatowski (Taxi)
Father Frank Dowling (Father Dowling Mysteries)
Father Merrin (The Exorcist)

Any more we should add? I nominate the Blues Brothers ("We're on a mission from God.").

Whoopie Goldberg and all the gals from "Sister Act". ;)

If they're going to add Father Dowling, we should add his sidekick Sister Steve (Tracy Nelson) as well.

Rev. Lovejoy (The Simpsons)

Reverend Camden-7th Heaven
Reverend Feltcher-All In The Family often mentioned but never seen.

Deacon Ernest Frye - Amen

John Michael Murphy - Father Murphy

Rev. Daniel Webster on the short lived tv show The Book Of Daniel.

Rev. Snow(Chrissy's father)-Three's Company

Rev. Mike Webber - Soul Man. In this short lived 1997 sitcom, Dan Aykroyd (SNL) played a widowed minister who drove a motorcycle and used unorthodox methods to get people into the church. I believe he did a crossover on Home Improvement as well.

Speaking of SNL - how could we forget Dana Carvey as the Church Lady?? :lol: